Kayven Riese kayve@sfsu.edu www.kayve.net 140 Steiner #6 San Francisco, CA 94117 (415) 902-5513 Education San Francisco State University, Computer Science Master's Degree candidate. Computing for Life Sciences Emphasis with Proteomic Techniques. City College of San Francisco, Computers and Information Systems 1998-2000, 2002-2003 MS MFC/C/C++ programming; MS Access; Oracle; Perl; Open/UNIX system administration; LAN; Physics with electromagnetism laboratory; HPLC; GC/MS. University of California at Berkeley- Extension 2001-2002 SAS; COM/DCOM; bioinformatics; MS dll programming. University of Southern California, Department of Physiology and Biophysics Master's Degree, 1995-1997 Conducted diabetes research on a SUN SPARC station IPX computer, UNIX based. Utilized SYNTAX (a metabolic simulator run on UNIX. Immunoprecipitation and western blot of insulin receptor (IR) transduction substrate for the insulin receptor (IRS-1) and inositol phosphate 3-kinase (IP3K) from insulin receptor transfected rat hepatoma. Sterile mammalian cell culture technique. Biostatistics course employing statistical software (STATA and SPSS) and ANOVA technique. University of California at Berkeley, Department of Molecular Cell Biology Molecular and Cell Neurobiology, 1991-1993 A self-designed independent study program: neuroanatomy, histophysiology, genetics, physiology (computer simulation laboratory), immunology, neurochemistry, neurobiology, cell biology, organic and biophysical chemistry, biochemistry (year lecture) and laboratory. University of Wisconsin at Madison, Departments of Mathematics and Computer Science Bachelor of Science (two full majors), 1985-1989 University of Wisconsin at Milwaukee Basic Science for Pre-Engineering, 1984-85 Economics, mathematics, chemistry, and physics, FORTRAN programming (EVAX); 39 units. Skills Molecular Biology: PCR, MALDI-TOF, 2D-PAGE Lowry/Bradford protein assay, SDS-PAGE, DNA/protein purification, RIA, sterile cell culture, Southern & Western blot, phage transduction, immunoprecipitation, cell receptor assay. Instrumentation: HPLC, UV spectrophotometry, light micrography, GC/MS, LSC/gamma radiotracer. Robotics: Hamilton and Beckman Mutimek 96 pin pipette automation; Carl Creative plate stacker. Web Development: HTML, CSS, XML, Perl, PHP, JavaScript, CGI, Java applet. Multimedia: Flash, GIMP, Photoshop, openGL, openAL, Corel Draw, ShowBiz. Bioinformatics: GenBank, BLAST, NCBI, SRS, PubMed, KEGG. DB/Spreadsheet: Oracle, MS Access, QBE, SQL, Quanta, QL, Excel, MySQL, Lotus, PL/SQL. Statistics: SigmaPlot, SAS, STATA, SPSS. OOP/PL/Script: VB, JAVA, C/C++, ksh, bash, FORTRAN, Pascal, Basic, AWK, LISP, OPS-5, MFC, JavaScript. OS: X, MS Windows (most types), EVAX, Linux, UNIX, MS-DOS. Assemblers: VAX11/780 and HP9000/300. Internet/Intranet: Pine, LAN, cc: email, (s)FTP, Novell Networking, kermit. Office/Presentation: MS Word, Microsoft Corel Draw, and Stella. Experience Oakland Unified School District, Day to day substitute 2006-present Chess Yoga (www.chessyoga.org), Webmaster 2004-present San Francisco State University, Disability Programs and Resource Center Student Assistant and Tutor, 2004-2005. University of California, San Francisco, Department of Ophthalmology Assistant. Richard B.. Crook, Ph.D. Pharmacology and Physiology Lab., 1994-1995, 2000-2003. Conducted research on isolated fetal human cell cultures of both PE and NPE using 86Rb+ uptake radioactive tracer assay. Linear regression fit conversion of raw LSC data according to isotope concentration specifications received from shipper. Utilized UV-sterilized biohazard containment hood for growth of experimental epithelial cell culture. Performed modified Lowry assay in the method of Peterson (Analytical Biochemistry 83, 346 (1977)). Nikon microphotographic system used for periodic assessment of viability of cultures. Volatile isotope 125I- efflux requiring radioactive-controlled hood was used as an analogue for Cl-. Utilized MedLine and PubMed health database search to assess the meaning of results and to aid experimental planning, e.g. in dosage and preincubation time decisions. Probed cells for NKCC protein utilizing denaturing 7.5% SDS-PAGE gels and western blot. Monteagle Medical Center 1998: Installed Windows 98 software, prepared medical presentations. Maintained contact information database. Acacia Biosystems, Inc. 1998. Implemented VB software to enable folder segmentation of Multimek scripts and 1536 colony omnitray no grow nomenclature facilitation utilizing LAN. Operated Multimek automated pipetter and Carl Creative plate stacker robots that required VB interface. Biocircuits Corporation Technical Administrator. Burlingame, CA. 1992, Hans Ribi. Organized biotechnological literature for scientific vice-president. Waisman Center Laboratory Assistant. Univ. of Wisconsin at Madison Medical School. 1989. High theoretical plate basic cleansing was performed, followed by autoclaving and high temperature baking to ensure that equipment was nucleic acid-free as well as sterile. Prepared solutions. Publications Lehman TD, Riese K, Lehman NL, Jackson PK, and Crook RB. Ubiquitination is involved in the regulation of Na-K-Cl cotransporter (NKCC) turnover in pigmented ciliary epithelial cells Investigative Ophthalmology and Visual Science 2007 48: E-5541. Available at www.iovs.org Riese K, Cohen DM, and Bergman RN. Stochastic properties of metabolites are dependent upon their own concentrations and enzymatic rates but not upon those of other metabolites, as calculated by SYNTAX. FASEB Supplement 11(3):A602, 3481 (Feb 1997) Riese K, Beyer AT, Lui GM, and Crook RB. Dopaminergic D1 stimulation of Na+,K+,Cl-cotransport in NPE cells: a role for multiple hormones. Investigative Ophthalmology and Visual Science 39: 1444-52(1998). Crook RB and Riese K. Beta-adrenergic stimulation of Na+,K+,Cl-cotransport in fetal nonpigmented ciliary epithelial cells. Investigative Ophthalmology and Visual Science 37:1047-1057(1996). Crook RB and Riese K. Adrenergic and dopaminergic regulation of Na+,K+,Cl- cotransport in human NPE cells. Experimental Eye Research 63:S24 (1996). Crook RB and Riese K. Protein phosphatases regulate Na+,K+,Cl-cotransport in fetal human NPE cells. Experimental Eye Research 63:S178(1996). Crook RB and Riese K. Protein phosphatases regulate Na+,K+,Cl-cotransport in fetal human NPE cells. Investigative Ophthalmology and Visual Science 37:S439 (1996). Riese K, Polansky JR, and Crook RB. Adrenergic stimulation of Na+,K+,Cl-cotransport in fetal NPE cells. Investigative Ophthalmology and Visual Science 36:S216 (1995).